Laboratory_for_the_diagnosis_of

      Laboratory of diagnostics of the state of the system of morphogenesis

The scientific direction of research is the study of the cell biology that express the marker proteins of epithelial stem and progenitor cells (cancer stem cells) when forming a transformed phenotype in the mouth cavity of people at risk of cancer (tobacco-smoking), in precancerous conditions, and progression to squamous cell carcinoma.
The proteomic study group studies changes in the expression profile of the p53, Ki-67, Bcl-2 and marker proteins of the p53, Cl5 / 14, CD44, ALDH1 biomarker proteins in the cellular layers of the squamous epithelium of the mucous membrane of the oral cavity in the formation of the transformed phenotype; epithelial-mesenchymal transition (EMT) in squamous cell carcinoma of the oral cavity during invasion and progression of the tumor process.
- Priority is to study the biology of the cells expressed in the marker proteins of the epithelial stem cells in the formation of the transformed phenotype in the oral mucosa of persons with a cancer risk (smoking habit), precancerous lesions and oral oral squamous cell carcinoma progression.
Proteome research group studies changes in the expression of the biomarker proteins p53, Ki-67, Bcl-2 and marker proteins of epithelial stem cells p63, Ck5 / 14, CD44, ALDHI, in the cell layers of the squamous epithelium of the oral mucosa in the formation of the transformed phenotype; and epithelial-mesenchymal transition (EMT) in oral squamous cell carcinoma (OSCC) in the invasion and progression of the tumor process.

                                         Projects implemented by the Laboratory:

"Investigation of the state of the epithelium of the mucous membrane of the mouth and the biology of basal cells in the formation of a transformed phenotype" (Implementation term 2007 - 2011)

                                                           Publications:

1. Kireeva SS, Lesnik IO, Yurchenko NP, Protsik VS, Sidorenko MV Expression of p53 and VEGF in patients with oral cancer // Journal of the Ear, Nose and Throat Diseases. - 2008. - No. 4. - p. 52-57.
2. Boroday NV, Sidorenko MV, Yurchenko N.P., Datsyuk I.O. Densiometric indices of nuclei of mucosal cells and tumor cells in histological sections of the epithelium biopsies at a distance from the tumor and tumor in patients with oral cancer. // Journal of the ear, nose and throat diseases. - 2008. - No. 4. - p. 6-10.
3. Kireeva SS, Protsik VS, Yurchenko N.P., Osadcha OM, Sidorenko MV Profile of proteins p53, Vsl-2, Ki-67 and changes in the epithelium of the mucosa at a distance from the formed tumor in patients with oral cancer. // Journal of the ear, nose and throat diseases. - 2010. - No. 4. - Page. 57-59.
4. Kireeva S. S., Yurchenko N.P., Ishchenko V.V., Skrypnikova T.P., Bashtan V.P. p53 and Ki-67 as biomarkers for the objectification of histological diagnosis of pre-tumor states and oral mucosal cancer on a biopsy material // Problems of ecology and medicine. - 2010. - t. 14, No. 3-4. - p. 17-21.
5. Kireeva SS, Lisnyak IO, Yurchenko NP, Protsik VS, Sidorenko MV Sensitivity to bleomycin mutagen of cultured lymphocytes and the state of the epithelium of the mucosa at a distance from the formed tumor in patients with oral cancer // Journal of the ear, nasal and throat diseases. - 2010. - No. 2. - Page. 7-12.
6. Kireeva S. S., Yurchenko N.P., Ishchenko V.V., Protsyk V.S., Sidorenko M.V. Changes in the mucosa at a distance from the formed tumor and the profile of the proteins p53 and Ki-67 in the cell layers of the epithelium of the mucosa in patients with squamous cell carcinoma of the cavity of the mouth // Problems of ecology and medicine. - 2010. - t. 14, No. 3-4. - page 17-21.
7. Kireeva S. S., Yurchenko N.P., Yakshibaeva Yu.R., Sidorenko M.V., Protsyk V.S. Investigation of the sensitivity of the body of the oral cavity cancer patient to the bleomycin mutagen and expression of p53 and the state of the epithelium of the mucosa at a distance from the developed tumor // Collection of scientific works of the VIII Congress of the Ukrainian Society of Genetics and Breeders of Ukraine named after them. E. Vavilov - Kyiv, Logos, 2007. - p. 455-459.
8. Osadcha O., Kireeva S., Yurchenko N. Alterations in the distant oral mucosa and buccal epithelium in Patients with OSCC and with epidemiological risk // 118th European Students Conference. 7-11 October 2007, Berlin, Germany. Medical faculty of humboldt university and free university of Berlin. P. 300
10. Kireeva S., Osadcha O., Sydorenko M. p53, Vsl-2, Ki-67, and alteration in tumor-distantoral mucosa in patients with oral squamous cell carcinoma (P34) // 5th internation conference cancer prevention. Advances in molecular and clinical aspects of cancer prevention. 6-8 March 2008, St. Gallen, Switzerland // Europ. J. Cancer. - 2008 vol. 6 (3). - P. 52
11. Kireeva S., Yurchenko N., Sydorenko M., Protsyk V. P63, CK14 and p53 expression in epithelial layers of tumor-distant oral mucosa in patients with oral squamous cell carcinoma (P7) // 6th internation conference on clinical cancer prevention. 18-20 March 2010, St. Gallen, Switzerland // Europ. J. Cancer. - 2010 - vol. 8 (2). - P. 12
12. Kireeva S. mutagen sensitivity and p53 in supragasal layers of tumor-distant oral mucosa in patient