Laboratory_of_morphogenetic_factors_of

          Laboratory of morphogenetic factors of microenvironment

Scientific direction of research: modeling and studying processes of formation of avascular tumor aggregates, interaction of tumor cells and elements of the immune system and stroma, increasing the effectiveness of antitumor therapy by modulating the conditions of the tumor microenvironment.
- Modeling and studying the formation of avascular tumor aggregates, the interaction of tumor cells and elements of the immune system and stroma, increasing the effectiveness of antitumor therapy by modulating the conditions of the tumor microenvironment.

Projects implemented by the Laboratory:

"Investigation of the possibilities of directed influence on cell morphogenesis in vitro" (Implementation term 2008-2011)
"Investigation of the possibilities of directed influence on cell morphogenesis in vitro" (Implementation term 2008-2011)

                                                         Publications:

1. Perepelitsina OM, Garmanchuk LV, Sidorenko MV Multicellular spheroids of breast cancer cells: conditions for the generation of MCF-7 and effects of serum factors // Bukovyna Medical Bulletin. 2007.-T.11, No. 3.- C.128-133.
2. Kokosyaz VM, Garmanchuk L.V., Nestorova OV, Yavorskaya NV, Senchilo NV, Pozur V.V. Patent for utility model "Application of acetate complex of copper and cadmium with ethylenediamine as an antitumor and anti-metastatic agent" No. 36261 dated October 27, 2008.
3. Perepelitsina O. M., Garmanchuk LV, Sidorenko MV Patent for utility model "Method of testing of biologically active compounds for their suitability as medicines" No. 35037, dated August 26, 08.
4. Perepelitsina OM, Garmanchuk LV, Kobelskaya OS, Sidorenko MV Influence of humoral factors produced by T-lymphocytes on proliferation and survival of MCF-7 cells // Immunology and Allergology. - 2008 - No. 2. - P.88-90.
5. Perepelitsyna O.M., Garmanchouk L.V., Sydorenko M.V., Ostapchenko L.I. Reactivity and sensitivity of breast adenocarcinoma (MCF-7) in coculture depends on the architecture of cell models // Physics of the Alive. - 2008 vol. 16, No. 2. - P. 105-111.

6. Garmanchuk LV, Perepelitsnaya EM, Sidorenko MV, Makarenko AN, Kulchikov AE The cytoprotective effect of neuropeptides on immunocompetent cells (in vitro study) // Experimental and clinical pharmacology. - 2009. - Vol. 79. - p. 28-31
7. Garmanchuk L.V., Perepelitsyna O.M., Sydorenko M.V., Ostapchenko L.I. Growth kinetics of 2- and 3-D cell models as influenced by microenvironment // Cytology and genetics. - 2009. - No. 5. - P. 305-309.
8. Kulchikov AE, Molozhaya O.S., Skachkova O.V., Garmanchuk L.V., Sidorenko MV, Perepelitsina EM, Khranovskaya N.N., Makarenko A.N. Comparative study of immunocormoning action of neuropeptide drugs in acute cerebrovascular pathology / / Cytokines and inflammation. - 2009. - No. 3. - Page 18-23.
9. Garmanchuk L.V., Perepelitsyna O.M., Sydorenko M.V., Ostapchenko L.I. Formation of Multicellular Aggregates under Different Conditions of Microenvironment // Cytology and Genetics. - 2010 - vol. 44, No. 1. - P. 19-22.
10. Perepelitsina OM, Garmanchuk L.V., Shevchenko Ye.A., Sidorenko MV, Ostapchenko L.I. Comparison of the level of NO products and proliferation of MCF-7 mammary carcinoma cells in the modification of microenvironment conditions // Ukr. Biochemical Journal. - 2010. - Vol. 82, No. 4. - P. 60-68.
11. Nepyvoda Kh.D., Garmanchuk LV, Perepelitsina OM, Skachkova O.V., Khranovskaya NM, Sidorenko MV, Ostapchenko L.I. Survival of 3-D culture of MCF-7 cells in the non-serum medium // Reports of the National Academy of Sciences of Ukraine. - 2011. - No. 8. - P. 160-164.
12. Garmanchuk L.V., Khranovskaya N.N., Makarenko AN, Mazur O.V., Skachkova O.V., Nepyvoda H. D., Lavrova E.V., Perepelitsina E.M., Senchilo NV, Vasilieva IG, Ostapchenko L.I. Comprehensive study of immunomodulatory effects of drugs with a neurotropic mechanism of action on immunocompetent cells // Zh. NAMS of Ukraine. - 2011. - 17, No. 3. - P. 218-226.
11. Perepelitsyna O.M., Garmanchouk L.V., Sydorenko M.V. Indirect effect of co-cultures MT4 on multicellular spheroids of breast adenocarcinoma (MCF7) // UICC, World-cancer congress, August 31, 2008, Geneva, Switzerland. [POS-B146].
12. Perepelitsyna E.M., Garmanchuk L.V., Kobelskaya E.S., Sidorenko M.V. Multi-directional influence of the K-medium of tumor lymphocytes on the proliferative activity of MCF-7 cells growing in 2-D and 3-D cultures // New cryobiotechnologies for the solution of fundamental and applied problems of medicine, November 26-28, 2008, Kharkiv, Ukraine.
13. Garmanchuk L.V. Perepelitsina EM, Sidorenko MV, Makarenko AN, Kulchikov AE Comparative study of the action of neuroprotectors on T-lymphocytes // Materials of the XVI Congress of the Russian National Congress "Man and Medicine", April 6-10, 2009, Moscow, Russia.
14. Garmanchuk LV, Perepelitsina EM, Sidorenko MV, Makarenko AN, Kulchikov AE Comparative study of the action of neuroprotectors on B-lymphocytes // Materials of the XVI Russian National Congress "Man and Medicine", April 6-10, 2009, Moscow, Russia.
15. Garmanchuk LV, Perepelitsyna EM, Nepivoda KD, Ostapchenko LI, Sidorenko MV Autocrine production of EPF-like polypeptides by tumor cells as a factor of their progression // Biologicheky active substances, fundamental and applied questions of obtaining and application, May 25, 2009, Novy Svet, Crimea, Ukraine. - Ct. 285-286.
16. Perepelitsyna O.M., Shevchenko EA, Skopenko O.V., Garmanchuk L.V., Alekseeva I.V., Palchikovskaya L.G., Sidorenko M.V. 6-azatsitytidine xylophuranoside inhibits the production of nitric oxide by the cancerous cells of MCF-7 // Biologicheky-active substances, fundamental and applied questions of obtaining and application, May 25, 2009, Novy Svet, AR Crimea, Ukraine .- Kr. 379-380.
17. Yavorskaya NV, Garmanchuk LV, Perepelitsina EM, Senchilo NV, Fursikova T.M., Nesterova O.V., Kokozey VN, Pozur V.K. Bactericidal, fungicidal and cytotoxic effects of heterobymetallic complexes // Biologicheky active substances, fundamental and applied questions of obtaining and application, May 25, 2009, Novy Svet, AR Crimea, Ukraine. - p. 451-452.
18. Perepelytsina O.M., Garmanchouk L.V., Sidorenko M.V., Kobelska O.S., Spivak N.J., Makarenko A.N. Immunotherapy and development of breast cancer. // Annals of Oncology. - 2009 - vol. 20, No. 2. - p.85.
19. Garmanchuk LV, Perepelitsina EM, Sidorenko MV, Makarenko AN, Kulchikov AE The cytoprotective effect of neuropeptides on immunocompetent cells (in vitro study) // Experimental and clinical pharmacology. - 2009. - 79, №4. - pp. 28-32.
20. Kobelskaya O.S., Zholobak N.M., Olenivska Z.M., Perepelitsina O.M., Maltsev G.V., Lyakhov S.A., Andronati S.A., Spivak M.Ya. Investigation of toxicity and interferonogenic action of derivatives of biphenyl in the culture of MT-4 cells // XII Congress of the Society of Microbiologists of Ukraine named after SMMVinogradsky, May 2009. - P. 192.
21. Perepelytsina O., Garmanchouk L., Sydorenko M., Ostapchenko L.I. The role of T-lymphocytes in the regulation of breast carcinogenesis in the model of multicellular
tumor sp
22. Perepelytsina O., Garmanchouk L., Sydorenko M., Gergeliuk T. Estrogen abrogated cytotoxic effect of tamoxifen on MCF-7 after activation of estrogen receptor by T-cells // ESMO-IMPAKT conference, May 2011, Brussels, Belgium.
t. Perepelytsina O., Sydorenko M., Skachkova O., Gergeliuk T. Methods for increasing titer of IFN in organism influence the effectiveness of hormonal therapy of breast cancer // Biologycally active substances: fundamental and applied problems, May 2011, Kiev Ukraine.
23. Garmanchuk L.V., Cheshuk V.E., Perepelitsina E.M., Sydorenko M.V. EGFR exspression and dimerization in the breast cancer resected postoperation tissue can be negative prognostic factor // Primary therapy of early breast cancer/The Breast: 11-th internat. confer., 11-14 March 2009.: abstract book.- St. Galen, Switzerlald, 2009. - [0042].-S.28.

"Investigation of pro- and anti-oncogenic activity of biological agents of cellular origin." (Implementation period 2011-2015)

                                                           Publications:
1. Indirect influence of co-cultures MT4 on multicellular spheroids of breast adenocarcinoma (MCF7); UICC, World-cancer congress, 31 August, 2008, Geneva [POS-B146]; Perepelitsyna O.M., Garmanchouk L.V., Sydorenko M.V.
2. Reactivity and sensibility of breast adenocarcinoma (MCF-7) in coculture depends on architecture of cells model; Physics of the Alive, t.16, No2, 2008; Perepelitsyna O.M., Garmanchouk L.V., Sydorenko M.V., Ostapchenko L.I.
3. EGFR expression and dimerization in the breast cancer resected postoperation tissue can be a negative prognostic factor; THE BREAST, Volume 18, Supplement 1, March 2009, P S28; Garmanchouk L.V.,O.M.Perepelytsina, V.E. Cheshuk, M.V. Sydorenko.
4. Growth Kinetics of 2- and 3-D Cell Models as Influenced by the Microenvironment; Cytology and genetics, 2009, Vol.43, No.5, P 305-309; L.V. Garmanchouk, O.M. Perepelytsina, M.V. Sidorenko, L.I. Ostapchenko.
5. Formation of Multicellular Aggregates under Different conditions of microenvironment; Cytology and genetics.- 2010.- Vol.44, №1.- P. 19-22; Perepelitsyna O.M., Garmanchouk L.V., Sydorenko M.V., Ostapchenko L.I.
6. Role of paracrine influence of T-lymphocytes in the regulation of the breast carcinogenesis on model of multicellular tumour spheroids; St.-Gallen Breast Cancer Conference, March, 2011; Abstract, poster presentation ; Perepelytsina O., Garmanchouk L., Sydorenko M., Ostapchenko L.I.
7. Estrogen abrogated cytotoxic effect of tamoxifen on MCF-7 after activation of estrogen receptor by T-cells; ESMO-IMPAKT conference, Brussels, May, 2011. Grand Susan G Commen for cure; Abstract, poster presentation; Perepelytsina O., Garmanchouk L., Sydorenko M., Gergeliuk T.
8. Perepelitsyna O. M., Gergelyuk T.S., Demianenko D., Goncharuk O., Golstev A., Sydorenko M.V. The receptor profile of breast tumor cells and formation of spheroids under influence of MSC // In this supplement primary therapy of early breast cancer 13 TH international conference st. Gallen, Switzerland, 13-16 march 2013.
9. Perepelitsyna O. M., Gergelyuk T.S., Demianenko D., Sydorenko M.V. Formation of multicellular tumor speroids by MCF-7 and sensitiviti to anticancer therapy // In this supplement primary therapy of early breast cancer 13-th international conference St. Gallen, Switzerland, 13-16 march 2013.
10. Т.С. Гергелюк, О.М. Перепелиціна, М.В. Сидоренко Порівняння впливу кондиційованого середовища від мезенхімальних стовбурових клітин людини та інтерферону-А на розвиток популяції пухлинних клітин MCF-7 // Буковинський медичний вісник. - 2014. - № 2 (70). - Стор. 18-23.
11.Demianenko D., Perepelytsina O., Yastrebova O., Sydprenko M. Effect of IFN-alpha and IFN-gamma on proliferation and receptor profile line of breast and colon cancer cells // Збірник наукових праць - International symposium on cell biology jointly with 4-th Ukrainian congress for cell biology. - 17-20.09.2014, Uzhhorod, Ukraine.

"Development of a cell test system for assessing the effectiveness and mechanisms of the influence of antitumor drugs" (Implementation period 2016-2020).

                                               Publications:
1. Perepelytsina O.M., Yakymchuk О., Vdovichenko N., Sydorenko M.V., Bakalinska O.N. Tumor spheroids as model for testing new anticancer drugs and not only // Enabling technologies in 3D cancer organoids, March 8-9, 2016, Torino, Italy.
2. Herheliuk Т., Perepelytsina О., Ostapchenko L., Yakymchuk O., Sydorenko M. Comparison of biological properties of C-medium from mesenchymal stem cells on 2D and 3D cells cultures of MCF-7 // Збірник тез XII Міжнародної наукової конференції студентів та аспірантів ”Молодь і поступ біології” - Львів, 25-27 квітня 2017, Україна. - стор. 243.

Functionalization of carbon nanomaterials surface by Doxorubicin and antibodies to tumor markers // Nanoscale Research Letters. - 2016. - 11(1):314. DOI: 10.1186/s11671-016-1537-z. 4. Ugnivenko A.P., Perepelitsina O.M., Sydorenko M.V., Ostapchenko L.I. Carbon nanotubes in delivery of bioactive substances // J. Bionanoscience. - 2017. - Vol. 11, № 6 (17). - P. 531-547. doi.org/10.1166/jbns.2017.1475 5. Nesterenko O.M., Herheliuk Т.S., Perepelytsina О.M., Sydorenko M.V., Bakalinska O.M., Ostapchenko L.I. Investigation of the degree of binding and controlled release of doxorubicin from the UDD and OLC // Proceedings of Ukranian Conference with Internetional participationn “Chemistry, physics and technology of surface” and Workshop “Nanostructured biocompatible/bioactive material”. - P. 112. 24-25 May, 2017. Kyiv, Ukrane. 6. Goncharuk O.V., Ugnivenko A.P., Terpilowski K., Skwarek E., Gun`ko V.M. Effect of ethonium adsorption on structure formation in nanosilica dispersions // // Proceedings of Ukranian Conference with Internetional participationn “Chemistry, physics and technology of surface” and Workshop “Nanostructured biocompatible/bioactive material”. - P. 66. 24-25 May, 2017. Kyiv, Ukrane. 7. Nesterenko O.M., Herheliuk Т.S., Perepelytsina О.M., Sydorenko M.V., Bakalinska O.M., Ostapchenko L.I. Investigation of the effectiveness of carbon nanotubes for targeted delivery of antitumor drugs, International research and practice conference: Nanotechnology and Nanomaterials (NANO-2017), 23-26 August, 2017, p. 636, Chernivtci, Ukrane.
3 Perepelytsina O., Garmanchouk L., Sydorenko M., Gergeliuk T. Estrogen abrogated cytotoxic effect of tamoxifen on MCF-7 after activation of estrogen receptor by T-cells // ESMO-IMPAKT conference, May 2011, Brussels, Belgium.
t Perepelytsina O., Sydorenko M., Skachkova O., Gergeliuk T. Methods for increasing the titer of IFN in the organism in the effect of the hormonal therapy of breast cancer // Biologycally active substances: Fundamental and Applied Problems, May 2011, Kiev Ukraine.
4. Garmanchuk L.V., Cheshuk V.E., Perepelitsina E.M., Sydorenko M.V. EGFR exspression and dimerization in a breast cancer resected postoperative tissue can be a negative prognostic factor // Primary therapy of early breast cancer / The Breast: 11th Internat. confer., 11-14 March 2009 .: abstract book.- St. Galen, Switzerlald, 2009. - [0042] .- S.28.

                                                           Publications:
1. Indirect effect of co-cultures MT4 on multicellular spheroids of breast adenocarcinoma (MCF7); UICC, World-cancer congress, August 31, 2008, Geneva [POS-B146]; Perepelitsyna O.M., Garmanchouk L.V., Sydorenko M.V.
2. Reactivity and sensitivity of breast adenocarcinoma (MCF-7) in coculture depends on the architecture of cell models; Physics of the Alive, t.16, No2, 2008; Perepelitsyna O.M., Garmanchouk L.V., Sydorenko M.V., Ostapchenko L.I.
3. EGFR expression and dimerization in a breast cancer resected postoperative tissue can be a negative prognostic factor; THE BREAST, Volume 18, Supplement 1, March 2009, P S28; Garmanchouk L.V., O.M.Perepelytsina, V.E. Cheshuk, M.V. Sydorenko.
4. Growth Kinetics of 2- and 3-D Cell Models as Influenced by the Microenvironment; Cytology and Genetics, 2009, Vol.43, No.5, P 305-309; L.V. Garmanchouk, O.M. Perepelytsina, M.V. Sidorenko, L.I. Ostapchenko.
5. Formation of Multicellular Aggregates under Different Conditions of Microenvironment; Cytology and Genetics.- 2010.- Vol.44, No. 1.- P. 19-22; Perepelitsyna O.M., Garmanchouk L.V., Sydorenko M.V., Ostapchenko L.I.
6. Role of paracrine influence of T-lymphocytes in the regulation of breast carcinogenesis on the model of multicellular tumor spheroids; St. Gallen Breast Cancer Conference, March, 2011; Abstract, poster presentation; Perepelytsina O., Garmanchouk L., Sydorenko M., Ostapchenko L.I.
7. Estrogen abrogated cytotoxic effect of tamoxifen on MCF-7 after activation of the estrogen receptor by T-cells; ESMO-IMPAKT conference, Brussels, May, 2011. Grand Susan G Commen for Cure; Abstract, poster presentation; Perepelytsina O., Garmanchouk L., Sydorenko M., Gergeliuk T.
8. Perepelitsyna O. M., Gergelyuk T. S., Demianenko D., Goncharuk O., Golstev A., Sydorenko M.V. The receptor profile of breast tumor cells and the formation of spheroids under the influence of MSC // In this supplementary primary therapy of early breast cancer 13 th international conference st. Gallen, Switzerland, March 13-16, 2013
9. Perepelitsyna O. M., Gergelyuk T. S., Demianenko D., Sydorenko M.V. Formation of Multicellular Nuclear Sphereids by MCF-7 and Sensitiviti Anticancer Therapy // In this supplement primary therapy for early breast cancer 13th international conference St. Gallen, Switzerland, March 13-16, 2013
10. T.S. Gergelyuk, O.M. Perepelitsina, MV Sidorenko Comparison of the effect of the conditioned medium on human mesenchymal stem cells and interferon-A on the development of the population of MCF-7 tumor cells // Bukovinsky medical bulletin. - 2014 - No. 2 (70). - P. 18-23
11.Demianenko D., Perepelytsina O., Yastrebova O., Sydprenko M. Effect of IFN-alpha and IFN-gamma on the proliferation and receptor profile of breast and colon cancer cells // Scientific Proceedings - International Symposium on Cell Biology jointly with 4th Ukrainian congress for cell biology. - 17-20.09.2014, Uzhhorod, Ukraine.

"Development of a cell test system for assessing the effectiveness and mechanisms of the influence of antitumor drugs" (Implementation period 2016-2020).

                                                 Publications:
1. Perepelytsina O.M., Yakymchuk O., Vdovichenko N., Sydorenko M.V., Bakalinska O.N. Tumor spheroids as model for testing new anticancer drugs and not only // Enabling technologies in 3D cancer organoids, March 8-9, 2016, Torino, Italy.
2. Herheliuk T., Perepelytsina O., Ostapchenko L., Yakymchuk O., Sydorenko M. Comparison of biological properties of C-medium from mesenchymal stem cells in 2D and 3D cell cultures of MCF-7 // Collection of theses of the XII International Scientific Conferences of students and postgraduates "Youth and Progress of Biology" - Lviv, April 25-27, 2017, Ukraine. - p. 243

"Testing of carbon-protein construct for the target delivery of antitumor drugs" of the target multidisciplinary multidisciplinary research program of the National Academy of Sciences of Ukraine "Molecular and cellular biotechnologies for the needs of medicine, industry and agriculture" and the order of the Presidium of the National Academy of Sciences of Ukraine