Department for Biotechnical Problems of Diagnostic
Відділення біотехічних проблем діагностики
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Веб-дизайн Анастасия Перепелицына perepelitsynaphotographer@gmail.com
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Дослідження про- та проти- онкогенної активності біологічних речовин клітинного походження
Дослідження впливу факторів клітинного походження на популяції стовбурових пухлинних клітин в культурі пухлинних мікросфероїдів
Тестування вуглецево-протеїнового конструкту для цільової доставки протипухлинних препаратів
«Розробка клітинної тест-системи для оцінки ефективності та механізмів впливу протипухлинних лікарських засобів»
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111P Effect of IFNa-2b on migrational ability of tumor cells on early stages
of breast cancer development

T. Herheliuk1, O. Perepelytsina1, L. Ostapchenko2, M. Sydorenko1
1Department of Biotechnical Problems of Diagnostics, Institute for problems of
Cryobiology & Cryomedicine, Kiev, Ukraine, 2Educational and Scientific Centre “Institute
of Biology & Medicine”, National University of Kyiv, Kyiv, Ukraine
Background: Inflammatory reactions play an important role in all stages of tumor development.

A shift to the mesenchymal phenotype causes an increase of migratory capacity
of tumor cells. Epithelial-mesenchymal transition (EMT) can also be caused by
local inflammation. Searching for factors that can inhibit the transition of the cell
population from the epithelial to the mesenchymal phenotype is very important for
antitumor therapy. Interferon alfa (IFNa-2b) can be such a factor that has a direct effect
on proliferation, differentiation and migration of tumor cells. The aim of this study was
to compare the effect of IFNa-2b on the expression of EMT markers and on the basic
cellular processes on 2D and 3D growth models.
Methods: 2Dcell culture was cultured in standard conditions withDMEMnutrientmedium(
Sigma,USA). The initial cell density was 2104 cells/cm2. Concentrations of
IFNa-2b were 103, 104 and 105 M/mL. For the initial generation of spheroids toDMEM
nutrient medium2%carboxymethylcellulose was added (Bio-Rad,USA). The spheroid
culture was maintained for 7 days on an orbital shaker (PSU-10i, Biosan, Latvia). Every
24 h cellswere counted in suspension and adhesion fractions using the routinemethod.
Cell viability was evaluated byMTT assay. The Stemi2000 software AxioVisionRed 4.7 was
used for image processing. The volume of spheroidswas calculated by Bjerkvig formula.
Markers were detected by applying IHC method with primarymonoclonal antibodies:Ck
(clone AE1/AE3,Dako,USA), vim (CloneV9,Dako,USA), EpCAM(Sigma,USA).
Results: The change of the cell growth type caused a change of the expression of some
EMTmarkers (CKs, EpCAM, Vim). IFNa-2b had a cytotoxic effect on tumor cells, and
decreased the migration ability. IFNa-2b caused an increasing of Ck and EpCAMexpression
by 50.5%and 47.8%, respectively, compared with control in 2D cell culture. In 3D
cell culture these increases were 33%and 34%, respectively, compared with the control.
Expression of vimsignificantly showed no difference compared with the control.
Conclusions: IFNa-2b stimulated the differentiation and inhibited a migrational ability
of tumor cells on early stages of breast cancer development.
Legal entity responsible for the study: Department of Biotechnical Problems of
Diagnostics, Institute for Problems of Cryobiology and Cryomedicine, NAS of Ukraine
Disclosure: All authors have declared no conflicts of interest.